Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars

@article{Perez1997CryopreservationAS,
  title={Cryopreservation and storage of embryogenic callus cultures of several Citrus species and cultivars},
  author={Rosa Maria Vega Perez and Luis Navarro and N{\'u}ria Duran-Vila},
  journal={Plant Cell Reports},
  year={1997},
  volume={17},
  pages={44-49}
}
Nucellus-derived embryogenic callus cultures of Salustiana sweet orange were subjected to cryoconservation assays. Cryoprotection with 10%(vol/vol) dimethylsulfoxide, freezing by slow cooling and thawing by fast warming was suitable to recover viable growing cultures and whole plants through embryogenesis. Evaluation of liquid phase R 1 and solid phase R 2 cooling rates using a programmable freezing unit indicated that 100% of embryogenic cultures survived when frozen using a range of cooling… CONTINUE READING
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