Cryopreservation and storage effects on cell numbers and DNA damage in human lymphocytes.

Abstract

The comet assay measures DNA damage in individual cells (usually lymphocytes) and is widely used in biomonitoring studies. Lymphocytes are harvested and are usually cryopreserved for batch testing. We investigated cell loss during harvesting, cryopreservation, thawing, and washing of human peripheral lymphocytes and compared DNA damage, using the Fpg… (More)
DOI: 10.1089/bio.2011.0024

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