Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations.

@article{Aisen2002CryopreservationAP,
  title={Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations.},
  author={Eduardo Gabriel Aisen and V H Medina and Andr{\'e}s Venturino},
  journal={Theriogenology},
  year={2002},
  volume={57 7},
  pages={1801-8}
}
We evaluated freeze-thawing tolerance of heterospermic ram spermatozoa (Pampinta breed) in a base diluent (Tris, citric acid, fructose, egg yolk, glycerol) with the addition of different trehalose concentrations (0-400 mOsm). We chose sperm motility, acrosome integrity and hypo-osmotic swelling test as parameters to evaluate cryopreservation capacity. We obtained the best results for 50 and 100 mOsm trehalose-supplemented extenders, with values (referred to fresh semen values) of 65% for… CONTINUE READING

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