Cryopreservable neutrophil surrogates. Stored cytoplasts from human polymorphonuclear leukocytes retain chemotactic, phagocytic, and microbicidal function.


Cryopreservation of polymorphonuclear leukocytes (PMN) has largely failed, probably because of their rich content of granular (lysosomal) enzymes. We have been developing granule-poor cytoplasts (anucleate fragments) from PMN which retain motile functions of the parent cell. The two types studied here were induced either by brief heating on surfaces… (More)