Cryoloading: introducing large molecules into live synaptosomes

  title={Cryoloading: introducing large molecules into live synaptosomes},
  author={Arup R. Nath and R. C. Chen and E. F. Stanley},
  journal={Frontiers in Cellular Neuroscience},
Neurons communicate with their target cells primarily by the release of chemical transmitters from presynaptic nerve terminals. The study of CNS presynaptic nerve terminals, isolated as synaptosomes (SSMs) has, however, been hampered by the typical small size of these structures that precludes the introduction of non-membrane permeable test substances such as peptides and drugs. We have developed a method to introduce large alien compounds of at least 150 kDa into functional synaptosomes… Expand
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