Cryoenzymologic studies on arginine kinase: solvent, temperature and pH effects on the overall reaction.

@article{Travers1978CryoenzymologicSO,
  title={Cryoenzymologic studies on arginine kinase: solvent, temperature and pH effects on the overall reaction.},
  author={Franck Travers and Richard Bertrand and Gilles Roseau and N van Thoai},
  journal={European journal of biochemistry},
  year={1978},
  volume={88 2},
  pages={523-8}
}
The overall reaction catalyzed by the phosphotransferase arginine kinase was studied at normal and subzero temperatures. Ethylene glycol was used as the antifreeze and its effects on the Km values of substances, kcat and pH profiles were investigated in detail. a) The Km values for the substrate (2 mM for ATP and 0.6 mM for arginine) were little affected by the solvent composition or temperature of the reaction mixture. b) At concentration of ethylene glycol higher than 40% there was a sharp… CONTINUE READING

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