Cryobiology of rat embryos II: A theoretical model for the development of interrupted slow freezing procedures.

@article{Liu2000CryobiologyOR,
  title={Cryobiology of rat embryos II: A theoretical model for the development of interrupted slow freezing procedures.},
  author={Jing-Yuan Liu and Erik J. Woods and Yuksel Agca and Elizabeth S Critser and John K. Critser},
  journal={Biology of reproduction},
  year={2000},
  volume={63 5},
  pages={1303-12}
}
Current mammalian embryo cryopreservation protocols typically employ an interrupted slow freezing (ISF) procedure. In general, ISF consists of initial slow cooling, which raises the extracellular solute concentration, and results in cell dehydration. Permeating cryoprotective agents (CPAs), such as dimethyl sulfoxide (DMSO), are typically included in the medium to protect the cells against high solute concentrations. As this ISF procedure continues, slow cooling is terminated at an intermediate… CONTINUE READING
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