Cryo-electron microscopy of viruses

@article{Adrian1984CryoelectronMO,
  title={Cryo-electron microscopy of viruses},
  author={Marc Adrian and Jacques Dubochet and Jean Lepault and Alasdair W. McDowall},
  journal={Nature},
  year={1984},
  volume={308},
  pages={32-36}
}
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare… Expand
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A novel approach to high-resolution ultrastructural analysis of cells and tissues based on the preparation of ultrathin frozen sections of fixed tissues, followed by their embedding on the grid in a layer of vitrified ice, and direct observation with a cryoelectron microscope is described. Expand
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This work has shown that preservation of bacteriophages in a thin layer of fast frozen buffer has proven to be a most convenient preparation method for obtaining images using cryo-electron microscopy (cryo-EM). Expand
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Ultrathin (70–100 nm thick) frozen‐hydrated cryosections of yeast cells were studied by scanning transmission electron microscopy (STEM). Generally, the contrast is very poor in brightfield and… Expand
1 Cryopreparation of Microorganisms for Electron Microscopy
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This chapter describes the cryopreparation of microorganisms for electron microscopy by means of cryofixation-based preparative procedures, which is mandatory for the correlation of structure and function at the ultrastructural level. Expand
Cryoelectron microscopy of vitrified Sendai virions.
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Morphology of vitrified Sendai virions was studied by transmission type electron microscopy, indicating pleomorphism of Sendaivirions in size but not in shape. Expand
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