Cryo-electron microscopy of viruses

  title={Cryo-electron microscopy of viruses},
  author={Marc Adrian and Jacques Dubochet and Jean Lepault and Alasdair W. McDowall},
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare… 

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A novel approach to high-resolution ultrastructural analysis of cells and tissues based on the preparation of ultrathin frozen sections of fixed tissues, followed by their embedding on the grid in a layer of vitrified ice, and direct observation with a cryoelectron microscope is described.

Cryo-Electron Microscopy of Viruses

How some of the key cryo-EM studies have advanced the authors' understanding of virus biology are reviewed and how the latest advances in cryoEM are described.

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The currently available and more commonly used protocols for rapid freezing such as: plunging, slamming, jet freezing, and high pressure freezing are illustrated and discussed shortly in the first section of this paper.



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It is shown that the contrast of T‐layer embedded in ice can be approximated to pure phase contrast.

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Thin layers of pure water or aqueous solutions are frozen in the vitreous state or with the water phase in the form of hexagonal or cubic crystals, either by using a spray‐freezing method or by

Electron Diffraction of Frozen, Hydrated Protein Crystals

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