Cryo-electron microscopy of viruses
@article{Adrian1984CryoelectronMO,
title={Cryo-electron microscopy of viruses},
author={Marc Adrian and Jacques Dubochet and Jean Lepault and Alasdair W. McDowall},
journal={Nature},
year={1984},
volume={308},
pages={32-36}
}Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare…
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