Cryo-electron microscopy of viruses

  title={Cryo-electron microscopy of viruses},
  author={M. Adrian and J. Dubochet and J. Lepault and A. McDowall},
  • M. Adrian, J. Dubochet, +1 author A. McDowall
  • Published 1984
  • Chemistry, Medicine
  • Nature
  • Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare… CONTINUE READING
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