Cryo-X-ray tomography of vaccinia virus membranes and inner compartments.

  title={Cryo-X-ray tomography of vaccinia virus membranes and inner compartments.},
  author={Jos{\'e} L Carrascosa and Francisco J Chich{\'o}n and Eva Pereiro and Mar{\'i}a Josefa Rodr{\'i}guez and J. J. Fern{\'a}ndez and M Carmen Desco Esteban and Stefan Heim and Peter Guttmann and Gerd Helge Schneider},
  journal={Journal of structural biology},
  volume={168 2},
Vitrified unstained purified vaccinia virus particles have been used as a test sample to evaluate the capabilities of cryo-X-ray tomography. Embedded in a thick layer of vitreous ice, the viral particles representing the mature form of the virus (MV) were visualized using full-field transmission X-ray tomography. The tomographic reconstructions reveal the viral brick-shaped characteristic structures with a size of 250x270x360nm(3). The X-ray tomograms show the presence of a clearly defined… CONTINUE READING