Cryo-EM visualization of an exposed RGD epitope on adenovirus that escapes antibody neutralization.

  title={Cryo-EM visualization of an exposed RGD epitope on adenovirus that escapes antibody neutralization.},
  author={Patrick L. Stewart and Charles Y Chiu and S Huang and Tobian Muir and Yingming Zhao and Bill Chait and Paula Mathias and Glen R. Nemerow},
  journal={The EMBO journal},
  volume={16 6},
Interaction of the adenovirus penton base protein with alpha v integrins promotes virus entry into host cells. The location of the integrin binding sequence Arg-Gly-Asp (RGD) on human type 2 adenovirus (Ad2) was visualized by cryo-electron microscopy (cryo-EM) and image reconstruction using a mAb (DAV-1) which recognizes a linear epitope, IRGDTFATR. The sites for DAV-1 binding corresponded to the weak density above each of the five 22 A protrusions on the adenovirus penton base protein… CONTINUE READING
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