Cryo-EM structure of a human spliceosome activated for step 2 of splicing

@article{Bertram2017CryoEMSO,
  title={Cryo-EM structure of a human spliceosome activated for step 2 of splicing},
  author={Karl Bertram and Dmitry E. Agafonov and Wen-ti Liu and Olexandr Dybkov and Cindy L Will and Klaus Hartmuth and Henning Urlaub and Berthold Kastner and Holger Stark and Reinhard L{\"u}hrmann},
  journal={Nature},
  year={2017},
  volume={542},
  pages={318-323}
}
Spliceosome rearrangements facilitated by RNA helicase PRP16 before catalytic step two of splicing are poorly understood. Here we report a 3D cryo-electron microscopy structure of the human spliceosomal C complex stalled directly after PRP16 action (C*). The architecture of the catalytic U2–U6 ribonucleoprotein (RNP) core of the human C* spliceosome is very similar to that of the yeast pre-Prp16 C complex. However, in C* the branched intron region is separated from the catalytic centre by… CONTINUE READING
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