Cryo-EM imaging of the catalytic subunit of the DNA-dependent protein kinase.

@article{Chiu1998CryoEMIO,
  title={Cryo-EM imaging of the catalytic subunit of the DNA-dependent protein kinase.},
  author={Charles Y. Chiu and Robert B. Cary and De Jie Chen and Scott R. Peterson and Phoebe L. Stewart},
  journal={Journal of molecular biology},
  year={1998},
  volume={284 4},
  pages={
          1075-81
        }
}
The DNA-dependent protein kinase (DNA-PK) plays an important role in mammalian DNA double-strand break repair and immunoglobulin gene rearrangement. The DNA-PK holoenzyme is activated by assembly at DNA ends and is comprised of DNA-PKcs, a 460 kDa protein kinase catalytic subunit, and Ku, a 70 kDa/80 kDa heterodimeric DNA-targeting component. We have solved the three-dimensional structure of DNA-PKcs to approximately 21 A resolution by analytically combining images of nearly 9500 individual… CONTINUE READING

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