Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation

@article{Bertram2017CryoEMSO,
  title={Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation},
  author={Karl Bertram and D. Agafonov and O. Dybkov and D. Haselbach and M. N. Leelaram and C. Will and H. Urlaub and B. Kastner and R. L{\"u}hrmann and H. Stark},
  journal={Cell},
  year={2017},
  volume={170},
  pages={701-713.e11}
}
  • Karl Bertram, D. Agafonov, +7 authors H. Stark
  • Published 2017
  • Biology, Medicine
  • Cell
  • Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially… CONTINUE READING
    119 Citations

    Figures and Topics from this paper.

    Explore Further: Topics Discussed in This Paper

    Structures of the human pre-catalytic spliceosome and its precursor spliceosome
    • 21
    • Highly Influenced
    • PDF
    Molecular architecture of the human 17S U2 snRNP
    • 2

    References

    SHOWING 1-10 OF 67 REFERENCES
    Cryo-EM structure of a human spliceosome activated for step 2 of splicing
    • 150
    • PDF
    Structure of a pre-catalytic spliceosome
    • 122
    • Highly Influential
    An Atomic Structure of the Human Spliceosome
    • 125
    Crystal structure of Prp8 reveals active site cavity of the spliceosome
    • 165
    A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity
    • 34
    • PDF
    Structure of a spliceosome remodelled for exon ligation
    • 105
    Structure of a yeast activated spliceosome at 3.5 Å resolution
    • 168
    • Highly Influential
    Molecular architecture of the human U4/U6.U5 tri-snRNP
    • 121
    • PDF
    Identification of a small molecule inhibitor that stalls splicing at an early step of spliceosome activation
    • 21