Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation

@article{Bertram2017CryoEMSO,
  title={Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation},
  author={Karl Bertram and Dmitry E. Agafonov and Olexandr Dybkov and David Haselbach and Majety Naga Leelaram and Cindy L Will and Henning Urlaub and Berthold Kastner and Reinhard L{\"u}hrmann and Holger Stark},
  journal={Cell},
  year={2017},
  volume={170},
  pages={701-713.e11}
}
Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially… CONTINUE READING
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