Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation

  title={Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation},
  author={Karl Bertram and D. Agafonov and O. Dybkov and D. Haselbach and M. N. Leelaram and C. Will and H. Urlaub and B. Kastner and R. L{\"u}hrmann and H. Stark},
  • Karl Bertram, D. Agafonov, +7 authors H. Stark
  • Published 2017
  • Biology, Medicine
  • Cell
  • Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially… CONTINUE READING
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