Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs.

@article{Banjar1983CrosslinkingOC,
  title={Crosslinking of chromosomal proteins to DNA in HeLa cells by UV gamma radiation and some antitumor drugs.},
  author={Zainy M. Banjar and L. S. Hnilica and R. C. Briggs and Juergen Stein and Gary S Stein},
  journal={Biochemical and biophysical research communications},
  year={1983},
  volume={114 2},
  pages={
          767-73
        }
}
Immunochemical techniques were used to investigate the protein-DNA crosslinking by ultraviolet (UV) and gamma radiation as well as 1,3-bis-(2-chloroethyl)-1-nitrosourea (BCNU) or cis- and trans-diamminedichloroplatinum II (cis-DDP and trans-DDP). Antisera to 0.35 M NaCl extract and 0.35 M NaCl residue of HeLa nuclei were employed. Both gamma and UV irradiation, exposure to cis- or trans-DDP and, to a lesser extent, BCNU, resulted in crosslinking of various antigens to the DNA. Although several… CONTINUE READING