Cross-talk-free simultaneous fluoroimmunoassay of two biomarkers based on dual-color quantum dots.

@article{Cao2011CrosstalkfreeSF,
  title={Cross-talk-free simultaneous fluoroimmunoassay of two biomarkers based on dual-color quantum dots.},
  author={Zhijuan Cao and Huan Li and Choiwan Lau and Yuhao Zhang},
  journal={Analytica chimica acta},
  year={2011},
  volume={698 1-2},
  pages={44-50}
}
In this article, we demonstrate the fabrication and simultaneous fluorescent detection of two biomarkers related to lung cancer. Polystyrene microspheres (PSM) were introduced as biomolecular immobilizing carriers and a 96-well filter plate was used as the separation platform. The whole experiment could be effectively carried out in a homogeneous system, as exemplified by the detection of carcinoembryonic antigen (CEA) and neuron specific enolase (NSE). First, two capture antibodies for CEA and… CONTINUE READING