Cross-species amplification of Megalobrama amblycephala EST-SSR in M.pellegrini and identification of their reciprocal hybrids

@inproceedings{Yi2013CrossspeciesAO,
  title={Cross-species amplification of Megalobrama amblycephala EST-SSR in M.pellegrini and identification of their reciprocal hybrids},
  author={Shaokui Yi and Zexia Gao and Wei Luo and Xiaoke Duan and Yanhe Li and Weimin Wang},
  year={2013}
}
To investigate the applicability of microsatellite markers developed from Megalobrama amblycephala in M.pellegrini and find the molecular markers to identify the M.amblycephala,M.pellegrini and their reciprocal hybrids,90 pairs of M.amblycephala EST-SSR primers were synthesized for cross-species amplification on the genome DNA of M.pellegrini,and 26 pairs of microsatellite primers of M.amblycephala and M.pellegrini were screened for PCR amplification on the genome DNA of M.amblycephala,M… CONTINUE READING

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