Cross-protection and selectable marker genes in plant transformation

  title={Cross-protection and selectable marker genes in plant transformation},
  author={Sung Hun Park and Susan C. Rose and Cecilia Zapata and Metinee Srivatanakul and Roberta H. Smith},
  journal={In Vitro Cellular & Developmental Biology - Plant},
Selectable marker genes play an important role in plant transformation. The level of selection pressure is generally established by generating a kill curve for the selectable marker. In most cases, the lowest concentration which kills all explants is used. This study examined two selectable marker genes, phosphinothricin acetyl transferase (PAT) and hygromycin phosphotransferase (HPT), in transformation of tobacco leaf disks. Experiments to determine the lethal level of the herbicide… CONTINUE READING