Cross-linking of protein crystals as an aid in the generation of binary protein-ligand crystal complexes, exemplified by the human PDE10a-papaverine structure.

@article{Andersen2009CrosslinkingOP,
  title={Cross-linking of protein crystals as an aid in the generation of binary protein-ligand crystal complexes, exemplified by the human PDE10a-papaverine structure.},
  author={Ole Andreas Andersen and Dorian Leo Sch{\"o}nfeld and Ian Toogood-Johnson and Brunella Felicetti and Claudia Albrecht and Tara Fryatt and Mark T. Whittaker and David Hallett and John L. Barker},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2009},
  volume={65 Pt 8},
  pages={872-4}
}
Protein crystallography has proven to be an effective method of obtaining high-resolution structures of protein-ligand complexes. However, in certain cases only apoprotein structures are readily available and the generation of crystal complexes is more problematic. Some crystallographic systems are not amenable to soaking of ligands owing to crystal-packing effects and many protein-ligand complexes do not crystallize under the same conditions as used for the apoprotein. Using crystals of human… CONTINUE READING