Cross-linking of intact erythrocyte membrane with a newly synthesized cleavable bifunctional reagent.

@article{Sato1981CrosslinkingOI,
  title={Cross-linking of intact erythrocyte membrane with a newly synthesized cleavable bifunctional reagent.},
  author={S. Sato and Makoto Nakao},
  journal={Journal of biochemistry},
  year={1981},
  volume={90 4},
  pages={
          1177-85
        }
}
  • S. SatoM. Nakao
  • Published 1 October 1981
  • Biology, Chemistry
  • Journal of biochemistry
In order to clarify the interaction of membrane proteins in intact cells (not ghosts), a new bifunctional cleavable reagent, maleimidomethyl-3-maleimido propionate (MMP), was synthesized. Human intact erythrocytes and ghosts were incubated with this reagent. Cross-linked products were analyzed by two-dimensional polyacrylamide gel electrophoresis with incubation of the first dimensional gel in 0.1 N NaOH for 15 min at room temperature before the second run. In the case of ghosts, newly appeared… 
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