Cross-linking of Novikoff ascites hepatoma cytokeratin filaments.

  title={Cross-linking of Novikoff ascites hepatoma cytokeratin filaments.},
  author={W Steven Ward and Warren N. Schmidt and Carla Adriana Pizarro Schmidt and L. S. Hnilica},
  volume={24 16},
We have investigated the structure of solubilized cytokeratins from Novikoff ascites hepatoma using the cleavable cross-linker 3,3'-dithiobis(sulfosuccinimidyl propionate) in the presence of 6 M urea to effect partial complex melting. By two-dimensional gel electrophoresis, in which the protein cross-links were broken in the second dimension, we have identified two major complexes as a p39-p56 dimer and a (p39-p56)2 tetramer, p39 and p56 being two of the major cytokeratins in Novikoff ascites… CONTINUE READING