Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.

@article{Cao2000CrosslinkedEA,
  title={Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.},
  author={Linqiu Cao and Fred van Rantwijk and Roger A Sheldon},
  journal={Organic letters},
  year={2000},
  volume={2 10},
  pages={1361-4}
}
[reaction--see text] Penicillin G acylase (penicillin amidohydrolase, E.C. 3.5.1.11) was immobilized in a simple and effective way by physical aggregation of the enzyme, using a precipitant, followed by chemical cross-linking to form insoluble cross-linked enzyme aggregates (CLEAs). These had the same activity in the synthesis of ampicillin as cross-linked crystals of the same enzyme, but the accompanying hydrolysis of the side-chain donor was much less. Penicillin G acylase CLEAs also… CONTINUE READING

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