Cross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces

@article{Macklis1985CrosslinkedCS,
  title={Cross-linked collagen surface for cell culture that is stable, uniform, and optically superior to conventional surfaces},
  author={Jeffrey D Macklis and Richard L. Sidman and Harold D. Shine},
  journal={In Vitro Cellular & Developmental Biology},
  year={1985},
  volume={21},
  pages={189-194}
}
SummaryA new type of collagen surface for use with cultures of peripheral nervous system cells is described. Collagen is derivatized to plastic culture dishes by a cross-linking reagent, 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide-metho-p-toluenesulfonate (carbodiimide), to form a uniform and durable surface for cell attachment and growth that allows dry storage, long-term culture, and improved microscopy. Surfaces of collagen derivatized to plastic were compared to surfaces of adsorbed or… CONTINUE READING
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