Cross-clade ultrasensitive PCR-based assays to measure HIV persistence in large-cohort studies.

@article{Vandergeeten2014CrosscladeUP,
  title={Cross-clade ultrasensitive PCR-based assays to measure HIV persistence in large-cohort studies.},
  author={Claire Vandergeeten and R{\'e}mi Fromentin and Esther Merlini and Mariam B. Lawani and Sandrina DaFonseca and Wendy Bakeman and Amanda McNulty and Moti N. Ramgopal and Nelson L Michael and Jerome H. Kim and Jintanat Ananworanich and Nicolas Chomont},
  journal={Journal of virology},
  year={2014},
  volume={88 21},
  pages={12385-96}
}
UNLABELLED A small pool of infected cells persists in HIV-infected individuals receiving antiretroviral therapy (ART). Here, we developed ultrasensitive assays to precisely measure the frequency of cells harboring total HIV DNA, integrated HIV DNA, and two long terminal repeat (2-LTR) circles. These assays are performed on cell lysates, which circumvents the labor-intensive step of DNA extraction, and rely on the coquantification of each HIV molecular form together with CD3 gene sequences to… CONTINUE READING
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