Cross-clade HIV-1 neutralizing antibodies induced with V3-scaffold protein immunogens following priming with gp120 DNA.

@article{ZollaPazner2011CrosscladeHN,
  title={Cross-clade HIV-1 neutralizing antibodies induced with V3-scaffold protein immunogens following priming with gp120 DNA.},
  author={Susan Zolla-Pazner and X-P Kong and Xunqing Jiang and Timothy Cardozo and Arthur N{\'a}das and Sandra C. M. Cohen and Maxim Totrov and Michael S. Seaman and Shixia Wang and Shan Lu},
  journal={Journal of virology},
  year={2011},
  volume={85 19},
  pages={9887-98}
}
The V3 epitope is a known target for HIV-1 neutralizing antibodies (NAbs), and V3-scaffold fusion proteins used as boosting immunogens after gp120 DNA priming were previously shown to induce NAbs in rabbits. Here, we evaluated whether the breadth and potency of the NAb response could be improved when boosted with rationally designed V3-scaffold immunogens. Rabbits were primed with codon-optimized clade C gp120 DNA and boosted with one of five V3-cholera toxin B fusion proteins (V3-CTBs) or with… CONTINUE READING