Cross Talk between Activation and Slow Inactivation Gates of Shaker Potassium Channels

@article{Panyi2006CrossTB,
  title={Cross Talk between Activation and Slow Inactivation Gates of Shaker Potassium Channels},
  author={Gyorgy Panyi and Carol Deutsch},
  journal={The Journal of General Physiology},
  year={2006},
  volume={128},
  pages={547 - 559}
}
This study addresses the energetic coupling between the activation and slow inactivation gates of Shaker potassium channels. To track the status of the activation gate in inactivated channels that are nonconducting, we used two functional assays: the accessibility of a cysteine residue engineered into the protein lining the pore cavity (V474C) and the liberation by depolarization of a Cs(+) ion trapped behind the closed activation gate. We determined that the rate of activation gate movement… CONTINUE READING