Cross-Linking the TCR Complex Induces Apoptosis in CD4+8+ Thymocytes in the Presence of Cyclosporin A

@article{Poetschke1996CrossLinkingTT,
  title={Cross-Linking the TCR Complex Induces Apoptosis in CD4+8+ Thymocytes in the Presence of Cyclosporin A},
  author={Heather L. Poetschke and David B. Klug and Dawn Walker and Ellen Rothman Richie},
  journal={Developmental Immunology},
  year={1996},
  volume={5},
  pages={1 - 15}
}
Although it is generally agreed that TCR ligation is a minimal requirement for negative selection in the CD4+8+ double-positive (DP) thymocyte subset, the costimulatory requirements and specific signaling events necessary to induce apoptosis are not well defined. We have explored the consequences of cross-linking CD3/TCR complexes on thymocytes from H-Y TCR transgenic (Tg) mice. In agreement with previous reports, we demonstrate that culturing DP thymocytes with plate-bound anti-TCR antibody… CONTINUE READING