Critical role of novel Thr-219 autophosphorylation for the cellular function of PKCtheta in T lymphocytes.

  title={Critical role of novel Thr-219 autophosphorylation for the cellular function of PKCtheta in T lymphocytes.},
  author={Nikolaus Thuille and Isabelle Heit and Friedrich Fresser and Nina Krumb{\"o}ck and Birgit Bauer and Sabine Leuthaeusser and Sascha Dammeier and Caroline Graham and Terry D. Copeland and Steve Shaw and Gottfried Baier},
  journal={The EMBO journal},
  volume={24 22},
Phosphopeptide mapping identified a major autophosphorylation site, phospho (p)Thr-219, between the tandem C1 domains of the regulatory fragment in protein kinase C (PKC)theta. Confirmation of this identification was derived using (p)Thr-219 antisera that reacted with endogenous PKCtheta in primary CD3+ T cells after stimulation with phorbol ester, anti-CD3 or vanadate. The T219A mutation abrogated the capacity of PKCtheta to mediate NF-kappaB, NF-AT and interleukin-2 promoter transactivation… CONTINUE READING
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