Critical role of calpain-mediated cleavage of calcineurin in excitotoxic neurodegeneration.

@article{Wu2004CriticalRO,
  title={Critical role of calpain-mediated cleavage of calcineurin in excitotoxic neurodegeneration.},
  author={Hai-yan Wu and Kazuhito Tomizawa and Yoshiya Oda and F Wei and Yun-fei Lu and Masayuki Matsushita and Sheng-tian Li and Akiyoshi Moriwaki and Hideki Asoh zToshihiro Matsui},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 6},
  pages={4929-40}
}
Calcineurin and calpain, a Ca2+/calmodulin-dependent protein phosphatase and a Ca2+-dependent cysteine protease, respectively, mediate neuronal cell death through independent cascades. Here, we report that during neuroexcitotoxicity, calcineurin A (CnA) is directly cleaved by calpain in vitro and in vivo, resulting in the enzyme being converted to an active form. Mass spectrometry identified three cleavage sites in CnA, two of which were constitutively active forms. Overexpression of the… CONTINUE READING