Critical role of c-Jun overexpression in liver metastasis of human breast cancer xenograft model

Abstract

c-Jun/AP-1 has been linked to invasive properties of aggressive breast cancer. Recently, it has been reported that overexpression of c-Jun in breast cancer cell line MCF-7 resulted in increased AP-1 activity, motility and invasiveness of the cells in vitro and tumor formation in nude mice. However, the role of c-Jun in metastasis of human breast cancer in vivo is currently unknown. To further investigate the direct involvement of c-Jun in tumorigenesis and metastasis, in the present study, the effects of c-Jun overexpression were studied in both in vitro and in nude mice. Ectopic overexpression of c-Jun promoted the growth of MCF-7 cells and resulted in a significant increase in the percentage of cells in S phase and increased motility and invasiveness. Introduction of c-Jun gene alone into weakly invasive MCF-7 cells resulted in the transfected cells capable of metastasizing to the nude mouse liver following tail vein injection. The present study confirms that overexpression of c-Jun contributes to a more invasive phenotype in MCF-7 cells. It indicates an interesting relationship between c-Jun expression and increased property of adhesion, migration and in vivo liver metastasis of MCF-7/c-Jun cells. The results provide further evidence that c-Jun is involved in the metastasis of breast cancer. The finding also opens an opportunity for development of anti-c-Jun strategies in breast cancer therapy.

DOI: 10.1186/1471-2407-7-145

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@article{Zhang2007CriticalRO, title={Critical role of c-Jun overexpression in liver metastasis of human breast cancer xenograft model}, author={Yan Zhang and Xiaoyun Pu and Ming Shi and Liyong Chen and Yuhua Song and Lu Qian and Guogang Yuan and Hao Zhang and Ming Yu and Meiru Hu and Beifen Shen and Ning Guo}, journal={BMC Cancer}, year={2007}, volume={7}, pages={145 - 145} }