Critical nucleotides in the upstream region of the XylS-dependent TOL meta-cleavage pathway operon promoter as deduced from analysis of mutants.

@article{GonzlezPrez1999CriticalNI,
  title={Critical nucleotides in the upstream region of the XylS-dependent TOL meta-cleavage pathway operon promoter as deduced from analysis of mutants.},
  author={M-Mar Gonz{\'a}lez-P{\'e}rez and Juan L. Ramos and Mar{\'i}a Trinidad Gallegos and Silvia Marqu{\'e}s},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 4},
  pages={2286-90}
}
The Pm promoter, dependent on TOL plasmid XylS regulator, which is activated by benzoate effectors, drives transcription of the meta-cleavage pathway for the metabolism of alkylbenzoates. This promoter is unique in that in vivo transcription is mediated by RNA-polymerase with different sigma factors. In vivo footprinting analysis shows that XylS interacted with nucleotides in the -40 to -70 region. In vivo and in vitro methylation of Pm shows extensive methylation of T at position -42 in the… CONTINUE READING

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