Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).

@article{DeSantis2003CreationOA,
  title={Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM).},
  author={Grace DeSantis and Kelvin H. L. Wong and Bob Farwell and Kelly Chatman and Zoulin Zhu and Geoff Tomlinson and Hongjun Huang and Xuqiu Tan and Lisa Bibbs and Pei Chung Chen and Keith A. Kretz and Mark J. Burk},
  journal={Journal of the American Chemical Society},
  year={2003},
  volume={125 38},
  pages={11476-7}
}
Gene site saturation mutagenesis (GSSM) technology is applied for the directed evolution of a nitrilase. The nitrilase effectively catalyzes the desymmetrization of the prochiral substrate 3-hydroxyglutaronitrile to afford (R)-4-cyano-3-hydroxybutyric acid, a precursor to the valuable cholesterol-lowering drug Lipitor. The discovered wild-type enzyme effectively performs the reaction at the industrially relevant 3 M substrate concentration but affords a product enantiomeric excess of only 87.6… CONTINUE READING

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