Creating small transcription activating RNAs.

  title={Creating small transcription activating RNAs.},
  author={James D. Chappell and Melissa K Takahashi and Julius B. Lucks},
  journal={Nature chemical biology},
  volume={11 3},
We expanded the mechanistic capability of small RNAs by creating an entirely synthetic mode of regulation: small transcription activating RNAs (STARs). Using two strategies, we engineered synthetic STAR regulators to disrupt the formation of an intrinsic transcription terminator placed upstream of a gene in Escherichia coli. This resulted in a group of four highly orthogonal STARs that had up to 94-fold activation. By systematically modifying sequence features of this group, we derived design… CONTINUE READING
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