Creating ribosomes with an all-RNA 30S subunit P site.

@article{Hoang2004CreatingRW,
  title={Creating ribosomes with an all-RNA 30S subunit P site.},
  author={Lee Hoang and Kurt Fredrick and Harry F. Noller},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 34},
  pages={12439-43}
}
Ribosome crystal structures have revealed that two small subunit proteins, S9 and S13, have C-terminal tails, which, together with several features of 16S rRNA, contact the anticodon stem-loop of P-site tRNA. To test the functional importance of these protein tails, we created genomic deletions of the C-terminal regions of S9 and S13. All of the tail deletions, including double mutants containing deletions in both S9 and S13, were viable, showing that Escherichia coli cells can synthesize all… CONTINUE READING