Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase.

@article{Groeve2009CreatingLP,
  title={Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase.},
  author={Manu R M de Groeve and Miet De Baere and Lieve Hoflack and Tom Desmet and Erick J M C Vandamme and Wim Soetaert},
  journal={Protein engineering, design & selection : PEDS},
  year={2009},
  volume={22 7},
  pages={393-9}
}
Disaccharide phosphorylases are interesting enzymes for the production of sugar phosphates from cheap starting materials and for the synthesis of novel glycosides. Cellobiose phosphorylase (CP) from Cellulomonas uda was subjected to directed evolution in order to create enzyme variants with significantly increased lactose phosphorylase (LP) activity, useful for the production of alpha-D-galactose 1-phosphate. In a first round, random mutagenesis was performed on part of the CP gene and the… CONTINUE READING