Cre-mediated recombination can induce apoptosis in vivo by activating the p53 DNA damage-induced pathway.

@article{Zhu2012CremediatedRC,
  title={Cre-mediated recombination can induce apoptosis in vivo by activating the p53 DNA damage-induced pathway.},
  author={Jianjian Zhu and M T Nguyen and Eiichiro Nakamura and Junming Yang and Susan Mackem},
  journal={Genesis},
  year={2012},
  volume={50 2},
  pages={102-11}
}
Cre-mediated apoptosis has been observed in many contexts in mice expressing Cre-recombinase and can confound the analysis of genetically engineered conditional mutant or transgenic alleles. Several mechanisms have been proposed to explain this phenomenon. We find that the degree of apoptosis induced correlates roughly with the copy number of loxP sites present in the genome and that some level of increased apoptosis accompanies the presence of even only a few loxP sites, as occurs in… CONTINUE READING