Cre-mediated germline mosaicism: a method allowing rapid generation of several alleles of a target gene.

Abstract

Conditional gene targeting uses the insertion of expression cassettes for the selection of targeted embryonic stem cells. The presence of these cassettes in the final targeted chromosomal locus may affect the normal expression of the targeted gene and produce interesting knock down phenotypes. We show here that the selection cassette may then be selectively… (More)

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