Cre-mediated generation of a VCAM-1 null allele in transgenic mice

@article{Terry1997CremediatedGO,
  title={Cre-mediated generation of a VCAM-1 null allele in transgenic mice},
  author={R W Terry and Lia Kwee and Harold Scott Baldwin and Mark A. Labow},
  journal={Transgenic Research},
  year={1997},
  volume={6},
  pages={349-356}
}
A conditional null allele for VCAM-1 was generated in mice through a one step ES cell selection procedure by flanking the proximal promoter and exons 1 and 2 with loxP sites. The ES cells were used to create chimeric mice, which were then used to produce mice homozygous for the VCAM-1 conditional null, or floxed allele. Although the PGKneo cassette was retained in the promoter, the homozygous mice produced levels of VCAM-1 transcripts similar to that seen in wild-type mice. Homozygous VCAMflox… CONTINUE READING
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