Cre-lox site-specific recombination between Arabidopsis and tobacco chromosomes.

@article{Koshinsky2000CreloxSR,
  title={Cre-lox site-specific recombination between Arabidopsis and tobacco chromosomes.},
  author={H A Koshinsky and Elizabeth Lee and David Ow},
  journal={The Plant journal : for cell and molecular biology},
  year={2000},
  volume={23 6},
  pages={715-22}
}
To create hybrid chromosomes, we tested the Cre-lox system to mediate recombination between Arabidopsis thaliana and Nicotiana tabacum chromosomes. Protoplasts of the two plants were fused to allow site-specific recombination to join a promoter from tobacco to a hygromycin resistance coding-region from Arabidopsis. The expected recombination junction was detected in hygromycin-resistant calli. Analysis of one hybrid suspension cell line revealed the presence of markers corresponding to the… CONTINUE READING