Cre-lox-regulated conditional RNA interference from transgenes.

  title={Cre-lox-regulated conditional RNA interference from transgenes.},
  author={Andrea Ventura and Alexander Meissner and Christopher P Dillon and Michael McManus and Phillip A. Sharp and Luk Van Parijs and Rudolf Jaenisch and Tyler 8 Jacks},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={101 28},
We have generated two lentiviral vectors for conditional, Cre-lox-regulated, RNA interference. One vector allows for conditional activation, whereas the other permits conditional inactivation of short hairpin RNA (shRNA) expression. The former is based on a strategy in which the mouse U6 promoter has been modified by including a hybrid between a LoxP site and a TATA box. The ability to efficiently control shRNA expression by using these vectors was shown in cell-based experiments by knocking… CONTINUE READING
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