Coxiella burnetii whole cell lysate protein identification by mass spectrometry and tandem mass spectrometry.

@article{Skultety2005CoxiellaBW,
  title={Coxiella burnetii whole cell lysate protein identification by mass spectrometry and tandem mass spectrometry.},
  author={Ludovit Skultety and Lenka Hernychov{\'a} and Rudolf Toman and Martin Hub{\'a}lek and Katar{\'i}na Slab{\'a} and Jana Zechovsk{\'a} and Veronika Stofan{\'i}kov{\'a} and Juraj Lenco and Jiř{\'i} Stul{\'i}k and Ales Macela},
  journal={Annals of the New York Academy of Sciences},
  year={2005},
  volume={1063},
  pages={115-22}
}
The whole cell lysate of Coxiella burnetii strain RSA 493 was separated by two-dimensional electrophoresis and more than 500 protein spots were found on silver-stained reference map. Spots from the gels were subjected to identification based on peptide mass fingerprinting (PMF). In order to identify additional proteins, tandem mass spectrometry (MS/MS) using electrospray and matrix-assisted laser desorption/ionization techniques was applied. The three independent approaches resulted in the… CONTINUE READING