Covalently linked peptides for enzyme-linked immunosorbent assay.

  title={Covalently linked peptides for enzyme-linked immunosorbent assay.},
  author={J. S\onderg{\aa}rd-Andersen and Edgar Lauritzen and Katrine Lind and Arne Holm},
  journal={Journal of immunological methods},
  volume={131 1},
A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detected by rabbit anti-angiotensin serum and with a positive serum from an HIV-2-infected patient, respectively, using the common ELISA procedure in the last steps. The method is simple to perform, it… CONTINUE READING