Covalently dimerized Camelidae antihuman TNFa single-domain antibodies expressed in yeast Pichia pastoris show superior neutralizing activity

@article{Ji2012CovalentlyDC,
  title={Covalently dimerized Camelidae antihuman TNFa single-domain antibodies expressed in yeast Pichia pastoris show superior neutralizing activity},
  author={Xuemei Ji and Wuguang Lu and Huiting Zhou and Dongju Han and Lin Yang and Haitao Wu and Jianfeng Li and Hongyan Liu and Jie Zhang and Peng Cao and Shuang-quan Zhang},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={97},
  pages={8547-8558}
}
Antagonists of tumor necrosis factor alpha (TNFa) have revolutionized the treatment of selected inflammatory diseases. Recombination Camelidae variable heavy-chain domain-only TNFa antibodies (anti-TNF-VHH) have been developed to antagonize the action of human and murine TNFa. Here, we describe a strategy to obtain functional covalent dimer anti-TNF-VHH molecules with the C-terminal fusion of human IgG1 Fc domain named anti-TNF-VHH-Fc. The resulting fusion proteins were separately expressed by… CONTINUE READING
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