Covalent immobilization of horseradish peroxidase via click chemistry and its direct electrochemistry.

@article{Ran2011CovalentIO,
  title={Covalent immobilization of horseradish peroxidase via click chemistry and its direct electrochemistry.},
  author={Qin Ran and Ru Fang Peng and Cong Liang and Siqiu Ye and Yuezhong Xian and Wenjing Zhang and Litong Jin},
  journal={Talanta},
  year={2011},
  volume={83 5},
  pages={1381-5}
}
A simple and versatile approach for covalent immobilization of redox protein on solid surface via self-assembled technique and click chemistry is reported. The alkynyl-terminated monolayers are obtained by self-assembled technique, then, azido-horseradish peroxidase (azido-HRP) was covalent immobilized onto the formed monolayers by click reaction. The modified process is characterized by reflection absorption infrared spectroscopy (RAIR), surface-enhanced Raman scattering spectroscopy (SERS… CONTINUE READING