Covalent immobilization of Drosophila acetylcholinesterase for biosensor applications.


The synthesized cDNA coding for AChE (acetylcholinesterase) was subcloned in pENTR/D-TOPO plasmid and expressed using baculovirus expression vector and Sf9 insect cells as host. Purified enzyme (specific activity 36374 micromol x min(-1) x mg(-1)) was immobilized on pre-activated perlite (a porous silica matrix) by silanization and glutaraldehyde treatment… (More)
DOI: 10.1042/BA20080005


  • Presentations referencing similar topics