Covalent binding of acidic drugs via reactive intermediates: detection of benoxaprofen and flunoxaprofen protein adducts in biological material.

@article{Dahms1996CovalentBO,
  title={Covalent binding of acidic drugs via reactive intermediates: detection of benoxaprofen and flunoxaprofen protein adducts in biological material.},
  author={Michael Dahms and Hildegard Spahn-Langguth},
  journal={Die Pharmazie},
  year={1996},
  volume={51 11},
  pages={874-81}
}
The purpose of the study was the direct detection of intact protein adducts-resulting from in-vitro incubations of flunoxaprofen- and benoxaprofen glucuronides in biological materials or originating from in vivo studies-by polyacrylamide gel electrophoresis (SDS-PAGE) followed by blotting and fluorescent scan, presumably yielding better specificity for the macromolecular binding partner and avoiding alkaline cleavage to release the aglycone. Glucuronides were isolated from urine samples or… CONTINUE READING