Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92.

@article{Fritzsche2003CovalentAT,
  title={Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92.},
  author={Hanna B Fritzsche and Torsten Schwede and Georg E. Schulz},
  journal={European journal of biochemistry},
  year={2003},
  volume={270 10},
  pages={
          2332-41
        }
}
Starting with oligopeptide sequences and using PCR, the gene of the cyclodextrinase from Flavobacterium sp. no. 92 was derived from the genomic DNA. The gene was sequenced and expressed in Escherichia coli; the gene product was purified and crystallized. An X-ray diffraction analysis using seleno-methionines with multiwavelength anomalous diffraction techniques yielded the refined 3D structure at 2.1 A resolution. The enzyme hydrolyzes alpha(1,4)-glycosidic bonds of cyclodextrins and linear… CONTINUE READING

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