Coupling of bradykinin receptors to phospholipase C in cultured fibroblasts is mediated by a G-protein.

@article{Etscheid1989CouplingOB,
  title={Coupling of bradykinin receptors to phospholipase C in cultured fibroblasts is mediated by a G-protein.},
  author={B G Etscheid and Mitchel L. Villereal},
  journal={Journal of cellular physiology},
  year={1989},
  volume={140 2},
  pages={264-71}
}
In cultured foreskin fibroblasts, bradykinin stimulates inositol phosphate generation, arachidonic acid release, and Na+/H+ exchange, with doses of 1-3 nM yielding half-maximal stimulation. Binding of 3H-bradykinin to these cells demonstrates a single receptor site with a Kd of 2.0 nM and a Bmax of 91 fmoles/mg protein. Bradykinin analogs of the B2 type inhibit this binding. GTP synergizes with bradykinin to stimulate phosphatidylinositol turnover in permeabilized fibroblasts and GTP-gamma-S… CONTINUE READING
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