Coupling of Ultrafast LC with Mass Spectrometry by DESI

@article{Cai2014CouplingOU,
  title={Coupling of Ultrafast LC with Mass Spectrometry by DESI},
  author={Yi Cai and Yong Liu and Roy Helmy and Hao Chen},
  journal={Journal of The American Society for Mass Spectrometry},
  year={2014},
  volume={25},
  pages={1820-1823}
}
  • Yi Cai, Y. Liu, +1 author H. Chen
  • Published 2014
  • Chemistry, Medicine
  • Journal of The American Society for Mass Spectrometry
AbstractRecently we reported a desorption electrospray ionization (DESI) interface to combine liquid chromatography (LC) with mass spectrometry (MS) using a new LC eluent splitting strategy through a tiny orifice on LC capillary tube [J. Am. Soc. Mass Spectrom. 25, 286 (2014)]. The interface introduces negligible dead volume and back pressure, thereby allowing “near real-time” MS detection, fast LC elution, and online MS-directed purification. This study further evaluates the LC/DESI-MS… Expand
Enhancing Performance of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry Using Trap and Capillary Columns.
TLDR
The data shows that liquid sample DESI-MS with a modified sample transfer capillary has significantly expanded its utility in bioanalysis. Expand
Integration of Electrochemistry with Ultra-Performance Liquid Chromatography/Mass Spectrometry
TLDR
This UPLC/EC/DESI-MS method combines the power of fast UPLC separation, fast electrochemical conversion, and online MS structural analysis for a potentially valuable tool for proteomics research and bioanalysis. Expand
Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry.
TLDR
The capabilities of LS-DESI extend and/or complement the utilities of traditional DESI and electrospray ionization and will find extensive and valuable analytical application in the future. Expand
Online Investigation of Aqueous-Phase Electrochemical Reactions by Desorption Electrospray Ionization Mass Spectrometry
TLDR
It was found that electroreduction of nitrosothiols leads to free thiols by loss of NO, as confirmed by online MS analysis for the first time, and saccharide oxidation, which parallels the oxidative metabolism observed for this drug in leucocytes, undergoes electro-oxidation to produce gluconic acid at alkaline pH, but not at neutral and acidic pHs. Expand
Online coupling techniques in ambient mass spectrometry.
TLDR
Close attention has been paid to different interface setups for coupling, as well as limits of detection, tolerance to different matrices, and applications of these new coupling techniques. Expand
Paper-Based Electrochemical Cell Coupled to Mass Spectrometry
TLDR
The results show that P-EC/SSI-MS has the ability to increase ionization efficiency, to perform online EC transformations, and to capture intermediates of EC reactions with a response time on the order of hundreds of milliseconds. Expand
Online Monitoring of Methanol Electro-Oxidation Reactions by Ambient Mass Spectrometry
TLDR
An online ambient mass spectrometric approach for analyzing HCHO generated from methanol electro-oxidation, taking the advantage of high salt tolerance of desorption electrospray ionization mass Spectrometry (DESI-MS) is presented. Expand
MS-Based Analytical Techniques: Advances in Spray-Based Methods and EI-LC-MS Applications
TLDR
A survey of some instrumental innovations and their applications with particular attention to spray-based MS methods and food analysis issues is provided. Expand
Ambient ionization MS for bioanalysis: recent developments and challenges.
TLDR
This review provides an introduction to the field and its application in bioanalysis, with an emphasis on the most recent developments and applications. Expand
Forensic Mass Spectrometry.
TLDR
This review attempts to capture the trends, motivating forces, and likely impact of developing areas of forensic mass spectrometry, with the caveat that none of this research is likely to have any real impact in the forensic community unless: (a) the instruments developed are turned into robust black boxes with red and green lights for positives and negatives, or (b) there are PhD graduates in the workforce who can help adopt these sophisticated techniques. Expand
...
1
2
...

References

SHOWING 1-10 OF 13 REFERENCES
A New Splitting Method for Both Analytical and Preparative LC/MS
TLDR
A novel splitting method for liquid chromatography/mass spectrometry (LC/MS) application, which allows fast MS detection of LC-separated analytes and subsequent online analyte collection and online derivatization using reactive DESI is possible for supercharging proteins and for enhancing their signals without introducing extra dead volume. Expand
Signal and Charge Enhancement for Protein Analysis by Liquid Chromatography-Mass Spectrometry with Desorption Electrospray Ionization.
TLDR
The use of desorption electrospray ionization (DESI) as a novel interface to couple high-performance liquid chromatography (HPLC) with mass spectrometry (MS) and the supercharging capability and the reduction of TFA signal suppression suggest that LC/DESI-MS is a valuable method for protein analysis. Expand
Investigations on the coupling of high-performance liquid chromatography to direct analysis in real time mass spectrometry.
TLDR
Experiments evaluating the correlation of signal intensity to mass-flow and concentration revealed that DART-MS can be seen as a mass flow sensitive detector. Expand
Direct analysis of liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS)
  • Z. Miao, H. Chen
  • Chemistry, Medicine
  • Journal of the American Society for Mass Spectrometry
  • 2009
TLDR
Results suggest that a charged droplet pick-up mechanism is responsible for desorption and ionization of liquid samples by DESI, which could have potentials in bioanalytical and forensic analysis. Expand
Metabolite identification using a nanoelectrospray LC-EC-array-MS integrated system.
A novel approach to the parallel coupling of normal-bore high-performance liquid chromatography (LC) with electrochemical-array detection (EC-array) and nanoelectrospray mass spectrometry (MS), basedExpand
New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches
TLDR
This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. Expand
Coupling of liquid chromatography with mass spectrometry by desorption electrospray ionization (DESI).
A liquid chromatography/mass spectrometry (LC/MS) method using desorption electrospray ionization (DESI) as a versatile interface has been established, which allows a wide range of elution flowExpand
On- and Off-Line Coupling of Separation Techniques to Ambient Ionization Mass Spectrometry
Li Li and Kevin A. Schug A review of recent research related to the coupling of ambient ionization mass spectrometry (MS) with various separation techniques is presented. Ambient ionization,Expand
Protonation in electrospray mass spectrometry: Wrong-way-round or right-way-round?
TLDR
Study of the dependence of ionization of the weak base caffeine on the electrospray capillary potential reveals three distinct contributors to wrong-way-round ionization. Expand
Protein structure evolution in liquid DESI as revealed by selective noncovalent adduct protein probing.
Previous experiments based on charge state distributions have suggested that liquid desorption electrospray ionization (DESI) is capable of preserving solution phase protein structure during transferExpand
...
1
2
...