Coupling of M(2) muscarinic receptors to Src activation in cultured canine colonic smooth muscle cells.

@article{Singer2002CouplingOM,
  title={Coupling of M(2) muscarinic receptors to Src activation in cultured canine colonic smooth muscle cells.},
  author={Cherie A. Singer and Sa Vang and William T. Gerthoffer},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  year={2002},
  volume={282 1},
  pages={G61-8}
}
The purpose of this study was to determine whether Src tyrosine kinases are one of the signaling intermediaries linking M(2) receptor stimulation to extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) in cultures of canine colonic smooth muscle cells (CSMC). RT-PCR studies demonstrate expression of multiple Src tyrosine kinases, including Src, Fyn, and Yes, in CSMC. Muscarinic stimulation of CSMC with 10 microM ACh results in a twofold increase in Src activity… CONTINUE READING