Coupling endonucleases with DNA end–processing enzymes to drive gene disruption

Abstract

Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this… (More)
DOI: 10.1038/nmeth.2177

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